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Issue: Vol. 5881 No. 3: 22 Sept. 2026
Type: Article
Published: 2026-09-22
DOI: 10.11646/zootaxa.5881.3.1
Page range: 301-326
Abstract views: 686
PDF downloaded: 11

A new species of Niceforonia (Anura: Craugastoridae) from the Colombian Massif, southwestern Colombia

Parque Nacional Natural Puracé; Dirección Territorial Andes Occidentales; Parques Nacionales Naturales de Colombia. Carrera 9 No. 25N – 06; Popayán; Cauca; Fundación Herencia Natural; Calle 32 EE # 80C-24; Medellín; Antioquia; Colombia
Programa Maestría en Tecnologías de la Información Geográfica; Facultad de Ciencias e Ingeniería; Universidad de Manizales; Carrera 9a # 19-03; Manizales; Caldas; Colombia
Departamento de Ciencias Biológicas; Facultad de Ciencias Exactas y Naturales; Universidad de Caldas; and Centro de Museos; Museo de Historia Natural; Universidad de Caldas. Calle 65 # 26-10; Manizales Caldas; Colombia
Fundación Ecohabitats; Calle 78N # 19 - 157 Casa 24 Portería 1; Popayán; Cauca; Colombia
Department of Ecology and Evolution; Biophore Building; University of Lausanne; 1015 Lausanne; Switzerland; Department of Ecology and Genetics; Evolutionary Biology; Norbyvägen 18D; 75236 Uppsala; Sweden; Laboratory of systematics and evolution; Biological faculty; V.N. Karazin National University; Svobody sq. 4; 61000; Kharkiv; Ukraine; Museum of Nature of the V.N. Karazin Kharkiv National University; Trinkler str. 8; 61000; Kharkiv; Ukraine
Wildlife Health Ghent; Faculty of Veterinary Medicine; Ghent University; Merelbeke; Belgium; Center for Conservation Genomics; Smithsonian Conservation Biology Institute; National Zoological Park; 3001 Connecticut Avenue NW; Washington; DC 20008; USA; CIBIO/InBIO; Centro de Investigação em Biodiversidade e Recursos Genéticos da Universidade do Porto; Campus Agrário de Vairão; 4485-661 Vairão; Portugal
Amphibia Amphibians Andes Páramo Puracé National Park Terrarana

Abstract

The genus Niceforonia currently includes 17 species of small, terrestrial, direct-developing frogs distributed from Colombia to northern Peru. In Colombia, 11 species of Niceforonia have been reported, with five of these restricted to high Andean ecosystems, including Páramos. Field surveys conducted between 2017 and 2023, in the Páramo ecosystems of the Puracé National Park and its buffer zone, in southwestern Colombia, revealed a previously unidentified species of Niceforonia. Through an integration of phylogenetic, ecological, bioacoustic, and morphological analyses, we identified unique traits that support the recognition of this population as a new species of Niceforonia, restricted to Páramo and Subpáramo habitats over 3300 m in the Colombian Massif. The new species is sister to Niceforonia brunnea, which occurs 195 kilometers further south in the Department of Nariño of southwestern Colombia and northern Ecuador. With the discovery of this new species, Colombia now hosts 12 species of Niceforonia, six of which inhabit Páramo ecosystems. Our study expands the taxonomic knowledge of the genus and underscores the importance of further exploration in high-Andean ecosystems of southwestern Colombia, where biodiversity remains underexplored.

References

  1. Acosta-Galvis, A.R. (2026) Lista de los Anfibios de Colombia. Version 16.2026. Available from: https://www.batrachia.com (accessed 13 January 2026)
  2. Acosta-Galvis, A.R., Streicher, J.W., Manuelli, L., Cuddy, T. & de Sá, R.O. (2018) Molecular insights into the phylogenetic placement of the poorly known genus Niceforonia Goin & Cochran, 1963 (Anura: Brachycephaloidea). Zootaxa, 4514 (4), 487–500. https://doi.org/10.11646/zootaxa.4514.4.3
  3. Agudelo-Hz, W.J., Urbina-Cardona, N. & Armenteras-Pascual, D. (2019) Critical shifts on spatial traits and the risk of extinction of Andean anurans: an assessment of the combined effects of climate and land-use change in Colombia. Perspectives in Ecology and Conservation, 17 (4), 206–219. https://doi.org/10.1016/j.pecon.2019.10.002
  4. Andersson, L.G. (1945) Batrachians from East Ecuador, collected 1937, 1938 by Wm. Clarke–Macintyre and Rolf Blomberg. Arkiv för Zoologi, Stockholm, 37A (2), 1–88.
  5. Angulo, A., Rueda-Almonacid, J.V., Rodríguez-Mahecha, J.V. & La Marca, E. (Eds.) (2006) Técnicas de inventario y monitoreo para los anfibios de la región tropical andina, Conservación Internacional. Serie Manuales de Campo Nº 2. Panamericana Formas e Impresos S.A., Bogotá, 299 pp.
  6. Anthelme, F. & Peyre, G. (2019) Biogeography of South American Highlands. In: Goldstein, M.I. & DellaSala, D.A. (Eds.), Encyclopedia of the world’s biomes. Vol. 1. Mountains (Alpine Systems) - Life at the Top. Elsevier, Amsterdam/Oxford, pp. 518–529. https://doi.org/10.1016/B978-0-12-409548-9.11811-1
  7. Ardila-Robayo, M.C., Ruiz-Carranza, P.M. & Barrera-Rodriguez, M. (1996) Una nueva especie de Phrynopus (Amphibia, Anura, Leptodactylidae) de la Cordillera Central Colombiana. Lozania, 67, 1–10.
  8. Batallas, D., Márquez, R. & Guayasamin, J.M. (2025) Sounds of the northern Andes: the calls of a diverse and endangered frog community (Amphibia, Anura) from Ecuador. ZooKeys, 1224, 211–252. https://doi.org/10.3897/zookeys.1224.137972
  9. Bonilla Valencia, J., Roncancio Duque, N. & Pisso-Florez, G.A. (2019) Portafolio de proyectos de investigación del Parque Nacional Natural Puracé. Documento adjunto al Plan de manejo 2020–2024 del PNP Puracé. Dirección Territorial Andes Occidentales. Parques Nacionales Naturales de Colombia, Popayán, Cauca, 58 pp.
  10. Bouckaert, R.R., Heled, J., Kühnert, D., Vaughan, T., Wu, C., Xie, D., Suchard, M.A., Rambaut, A. & Drummond, A.J. (2014) BEAST 2: A Software Platform for Bayesian Evolutionary Analysis. Plos computational biology, 10, e1003537. https://doi.org/10.1371/journal.pcbi.1003537
  11. Bouckaert, R.R. & Drummond, A.J. (2017) bModelTest: Bayesian phylogenetic site model averaging and model comparison. BMC Evolutionary biology, 17, 42. https://doi.org/10.1186/s12862-017-0890-6
  12. Boulenger, G.A. (1882) Catalogue of the Batrachia Salientia s. Ecaudata in the Collection of the British Museum. 2nd Edition. Taylor and Francis, London, xvi + 503 pp. https://doi.org/10.5962/bhl.title.54882
  13. Boulenger, G.A. (1908) Descriptions of new batrachians and reptiles discoverd by Mr. M.G. Palmer in south-western Colombia. Annals and Magazine of Natural History, 8 (2), 515–522. https://doi.org/10.1080/00222930808692531
  14. Buitrago-González, W., López-Guzmán, J.H. & Vargas-Salinas, F. (2016) Anuros en los complejos paramunos Los Nevados, Chilí–Barragán y Las Hermosas, Andes centrales de Colombia. Biota Colombiana, 17 (2), 52–76. https://doi.org/10.21068/c2016v17s02a04
  15. Buitrago-González, W., López-Guzmán, J.H. & Vargas-Salinas, F. (2016) Niceforonia adenobrachia Ardila-Robayo, Ruiz-Carranza & Barrera-Rodríguez, 1996 (Amphibia: Anura: Craugastoridae): extension of geographical distribution in the Central Andes of Colombia. Check List, 12 (1), 1–3. https://doi.org/10.15560/12.1.1845
  16. Camacho, C., Coulouris, G., Avagyan, V., Man, N., Papadopoulos, J., Bealer, K. & Madden, T.L. (2009) BLAST+: architecture and applications. BMC Bioinformatics, 10, 421. https://doi.org/10.1186/1471-2105-10-421
  17. Cannatella, D.C. (1984) Two new species of the leptodactylid frog genus Phrynopus, with comments on the phylogeny of the genus. Occasional Papers of the Museum of Natural History, University of Kansas, 113, 1–16.
  18. Carrión-Olmedo, J.C. & Ron, S.R. (2021) A new species of the Pristimantis lacrimosus group (Anura, Strabomantidae) from the eastern slopes of the Ecuadorian Andes. Evolutionary Systematics, 5, 151–175. https://doi.org/10.3897/evolsyst.5.62661
  19. Christmann, T. & Oliveras, I. (2020) Nature of alpine ecosystems in tropical mountains of South America. In: Goldstein, M.I. & DellaSala, D.A. (Eds.), Encyclopedia of the world’s biomes. Vol. 1. Mountains (Alpine Systems) - Life at the Top. Elsevier, Amsterdam/Oxford, pp. 282–291. https://doi.org/10.1016/B978-0-12-409548-9.12481-9
  20. Crump, M.L. & Scott, N.J. (1994) Standard techniques for inventory and monitoring: visual encounter surveys. In: Heyer, R.W., Donnelly, M.A., McDiarmid, R.W., Hayek, L.A. & Foster, M.S. (Eds.), Measuring and Monitoring Biological Diversity. Standard Methods for Amphibians. Smithsonian Institution Press, Washington, D.C., pp. 84–92.
  21. Cuatrecasas, J. (1958) Aspectos de la vegetación natural de Colombia. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 10 (40), 221–268. https://doi.org/10.18257/raccefyn.3462
  22. Darst, C.R. & Cannatella, D.C. (2004) Novel relationships among hyloid frogs inferred from 12S and 16S mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 31, 462–475. https://doi.org/10.1016/j.ympev.2003.09.003
  23. De Queiroz, K. (2007) Species concepts and species delimitation. Systematic biology, 56, 879–886. https://doi.org/10.1080/10635150701701083
  24. Duellman, W.E. (2000) Leptodactylid frogs of the genus Phrynopus in northern Peru with descriptions of three new species. Herpetologica, 56, 273–285.
  25. Duellman, W.E. & Pramuk, J.B. (1999) Frogs of the genus Eleutherodactylus (Anura: Leptodactylidae) in the Andes of northern Peru. Scientific Papers. Natural History Museum, University of Kansas, 13, 1–78. https://doi.org/10.5962/bhl.title.16169
  26. Duellman, W.E. & Lehr, E. (2009) Terrestrial breeding frogs (Strabomantidae) in Peru. Lonnemann GmbH, Selm, Berlin, 382 pp.
  27. Emmrich, M., Vences, M., Ernst, R., Köhler, J., Barej, M.F., Glaw, F., Jansen, M. & Rödel, M.O. (2020) A guild classification system proposed for anuran advertisement calls. Zoosystematics and Evolution, 96 (2), 515–525. https://doi.org/10.3897/zse.96.38770.figure1
  28. Frost, D.R. (2026) Amphibian Species of the World: an Online Reference. Version 6.2. American Museum of Natural History, New York, New York. Available from: https://amphibiansoftheworld.amnh.org/index.php (accessed 13 March 2026) https://doi.org/10.5531/db.vz.0001
  29. Goin, C.J. & Cochran, D.M. (1963) Two new genera of leptodactylid frogs from Colombia. Proceedings of the California Academy of Sciences, 4 (31), 499–505.
  30. Hay, J.M., Ruvinsky, I., Hedges, S.B. & Maxson, L.R. (1995) Phylogenetic relationships of amphibian families inferred from DNA sequences of mitochondrial 12S and 16S ribosomal RNA genes. Molecular Biology and Evolution, 12, 928–937. https://doi.org/10.1093/oxfordjournals.molbev.a040270
  31. Hedges, S.B., Duellman, W.E. & Heinicke, M.P. (2008) New World direct-developing frogs (Anura: Terrarana): molecular phylogeny, classification, biogeography, and conservation. Zootaxa, 1737 (1), 1–182. https://doi.org/10.11646/zootaxa.1737.1.1
  32. Heinicke, M.P., Duellman, W.E. & Hedges, S.B. (2007) Major Caribbean and Central American frog faunas originated by ancient oceanic dispersal. PNAS, 104 (24), 10092–10097. https://doi.org/10.1073/pnas.0611051104
  33. Hofstede, R., Llambí, L., Peralvo, M., Beltrán, K., Calispa, M. & Mosquera, G. (2023) El cambio climático en el páramo del Ecuador. In: Mena-Vásconez, P., Hofstede, R. & Suárez Robalino, E. (Eds.), Los páramos del Ecuador: Pasado, presente y futuro. USFQ PRESS, Quito, pp. 324–353. https://doi.org/10.18272/usfqpress.71.c268
  34. IUCN (2025) The IUCN Red List of Threatened Species. Version 2023-1. Available from: https://www.iucnredlist.org (accessed 13 August 2026)
  35. Jiménez de la Espada, M. (1870) Fauna neotropicalis species quaedam nondum cognitae. Jornal de Sciências, Mathemáticas, Physicas e Naturaes, 3, 57–65.
  36. Jiménez de la Espada, M. (1875) Vertebrados del Viaje al Pacífico Verificado de 1862 a 1865 por una Comisión de Naturalistas Enviada por el Gobierno Español. Batracios. A. Miguel Ginesta, Madrid, 208 pp. https://doi.org/10.5962/bhl.title.5769
  37. K. Lisa Yang Center for Conservation Bioacoustics at the Cornell Lab of Ornithology. (2024) Raven Pro: Interactive Sound Analysis Software. Version 1.6.5. Computer software. The Cornell Lab of Ornithology, Ithaca, New York. Available from: https://www.ravensoundsoftware.com/ (accessed 13 August 2026)
  38. Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
  39. Köhler, J., Jansen, M., Rodriguez, A., Kok, P.J., Toledo, L.F., Emmrich, M., Glaw, F., Haddad, C.F.B., Rödel, M.O. & Vences, M. (2017) The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa, 4251 (1), 1–124. https://doi.org/10.11646/zootaxa.4251.1.1
  40. Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35, 1547–1549. https://doi.org/10.1093/molbev/msy096
  41. Lynch, J.D. (1973) A new narrow-toed frog from Andean Ecuador (Leptodactylidae: Eleutherodactylus). Copeia, 1973 (2), 222–225. https://doi.org/10.2307/1442960
  42. Lynch, J.D. (1975) A review of the Andean leptodactylid frog genus Phrynopus. Occasional Papers of the Museum of Natural History University of Kansas, 35, 1–51.
  43. Lynch, J.D. (1980) New species of Eleutherodactylus of Colombia (Amphibia: Leptodactylidae). I: five new species from the paramos of the Cordillera Central. Caldasia, 13, 165–188.
  44. Lynch, J.D. (1989) Intrageneric relationships of mainland Eleutherodactylus (Leptodactylidae). I. A review of the frogs assigned to the Eleutherodactylus discoidalis species group. Contributions in Biology and Geology Milwaukee Public Museum, 79, 1–25.
  45. Lynch, J.D. (2003) A new species of frog from northeastern Colombia (genus Eleutherodactylus: Leptodactylidae). Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 27, 287–289. https://doi.org/10.18257/raccefyn.27(103).2003.2069
  46. Lynch, J.D. & Ruiz-Carranza, P.M. (1983) New frogs of the genus Eleutherodactylus from the Andes of southern Colombia. Transactions of the Kansas Academy of Science, 86, 99–112. https://doi.org/10.2307/3628163
  47. Lynch, J.D. & Duellman, W.E. (1980) The Eleutherodactylus of the Amazonian slopes of the Ecuadorian Andes (Anura: Leptodactylidae). The University of Kansas, Museum of Natural History, Miscellaneous Publications, 69, 1–86. https://doi.org/10.5962/bhl.title.16222
  48. Lynch, J.D. & Duellman, W.E. (1997) Frogs of the genus Eleutherodactylus in western Ecuador. Systematics, ecology, and biogeography. Special Publication. Natural History Museum, University of Kansas, 23, 1–236. https://doi.org/10.5962/bhl.title.7951
  49. Lynch, J.D. & Suárez-Mayorga, A. (2002) Análisis biogeográfico de los anfibios paramunos. Caldasia, 24, 471–480. [https://www.jstor.org/stable/23641602]
  50. Morales, M., Otero, J., Van der Hammen, T., Torres, A., Cadena, C., Pedraza, C., Rodríguez, N., Franco, C., Betancourth, J.C., Olaya, E., Posada, E. & Cárdenas, L. (2007) Atlas de páramos de Colombia. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Bogotá, D.C., 208 pp.
  51. Mueses-Cisneros, J.J. (2005) The Amphibian Fauna of the Valle de Sibundoy, Putumayo–Colombia. Caldasia, 27, 229–242.
  52. Mueses-Cisneros, J.J. & Yánez-Muñoz, M.H. (2009) Técnica de Remoción con Rastrillo y Azadón (RRA): una metodología adecuada para una fauna inadecuadamente muestreada, la Herpetofauna de la Región Paramuna. In: Vriesendorp, C., Alverson, W.S., Del Campo, Á., Stotz, D.F., Moskovits, D.K., Fuentes Caceres, S., Coronel Tapia, B. & Anderson, E.P. (Eds.), Ecuador: Cabeceras Cofanes–Chingual, Rapid Biological and Social Inventories Report 21. The Field Museum Chicago Museum, Chicago, pp. 284–287.
  53. Ospina-Sarria, J.J. & Duellman, W.E. (2019) Two new Species of Pristimantis (Amphibia: Anura: Strabomantidae) from southwestern Colombia. Herpetologica, 75, 85–95. [https://www.jstor.org/stable/27098782] https://doi.org/10.1655/d-18-00019
  54. Ospina-Sarria, J.J. & Grant, T. (2022) New phenotypic synapomorphies delimit three molecular-based clades of New World direct-developing frogs (Amphibia: Anura: Brachycephaloidea). Zoological Journal of the Linnean Society, 195, 976–994. https://doi.org/10.1093/zoolinnean/zlab071
  55. Padial, J.M., Chaparro, J.C. & de la Riva, I. (2008) Systematics of Oreobates and the Eleutherodactylus discoidalis species group (Amphibia, Anura), based on two mitochondrial DNA genes and external morphology. Zoological Journal of the Linnean Society, 152 (4), 737–773. https://doi.org/10.1111/j.1096-3642.2007.00372.x
  56. Padial, J.M., Grant, T. & Frost, D. (2014) Molecular systematics of terraranas (Anura: Brachycephaloidea) with an assessment of the effects of alignment and optimality criteria. Zootaxa, 3825 (1), 1–132. https://doi.org/10.11646/zootaxa.3825.1.1
  57. Peracca, M.G. (1904) Viaggio del Dr. Enrico Festa nell’Ecuador e regioni vicine. Rettili ed anfibi. Bollettino dei Musei di Zoologia ed Anatomia Comparata della R. Università di Torino, 19 (465), 1–41 https://doi.org/10.5962/bhl.part.11596
  58. Pisso-Florez, G.A., Liévano-Bonilla, A.F., Mazepa, G., Mulder, K.P. & Ramírez-Chaves, H.E. (2023a) Distribution Update, Advertisement Call, and the Phylogenetic Position of the Malvasá Rocket Frog, Hyloxalus pinguis (Anura: Dendrobatidae). The South American Journal of Herpetology, 28 (1), 78–88. https://doi.org/10.2994/SAJH-D-22-00002.1
  59. Pisso-Florez, G.A., Lievano-Bonilla, A.F., Mendoza-Henao, A.M. & Duarte-Marín, S. (2023b) The distress call and range extension of the Hermosas Robber Frog, Pristimantis racemus (Anura: Strabomantidae). Revista Latinoamericana de herpetología, 6 (1), 23–31. https://doi.org/10.22201/fc.25942158e.2023.01.560
  60. Pyron, R.A. & Wiens, J.J. (2011) A large-scale phylogeny of Amphibia including over 2800 species, and a revised classification of advanced frogs, salamanders, and caecilians. Molecular Phylogenetics and Evolution, 61, 543–583. https://doi.org/10.1016/j.ympev.2011.06.012
  61. Rangel-Ch, J.O. (2000) Colombia diversidad biotica III. La región de vida paramuna. Universidad Nacional de Colombia, Facultad de Ciencias, Instituto de Ciencias Naturales & Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Bogotá, 866 pp.
  62. R Core Team. (2023) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. Available from: https://www.r-project.org (accessed 20 August 2023)
  63. Reyes-Puig, J.P., Reyes-Puig, C., Ron, S.R., Ortega, J.A., Guayasamin, J.M., Goodrum, M., Recalde, F., Vieira, J.J., Koch, C. & Yánez-Muñoz, M.H. (2019) A new species of terrestrial frog of the genus Noblella Barbour, 1930 (Amphibia: Strabomantidae) from the Llanganates-Sangay Ecological Corridor, Tungurahua, Ecuador. PeerJ, 7, e7405. https://doi.org/10.7717/peerj.7405
  64. Reyes-Puig, C., Yánez-Muñoz, M.H., Ortega, J.A. & Ron, S.R. (2020) Phylogenetic relationships of the subgenus Hypodictyon (Anura: Strabomantidae: Pristimantis) with the description of three new species from the Chocó región. Revista Mexicana de Biodiversidad, 91, e913013. https://doi.org/10.22201/ib.20078706e.2020.91.3013
  65. Reyes-Puig, J.P., Yánez-Muñoz, M.H., Urgilés-Merchán, M., Libke, Z., Ron, S.R. & Carrión-Olmedo, J.C. (2026) Two new terrestrial narrow-toed frogs (Niceforonia: Strabomantidae, Anura) from remote unexplored mountains in the Ecuadorian Andes. PeerJ, 14, e21182. https://doi.org/10.7717/peerj.21182
  66. Rivera, D. & Rodríguez, C. (2011) Guía Divulgativa de Criterios Para La Delimitación de Páramos de Colombia. Ministerio de Ambiente, Vivienda y Desarrollo Territorial & Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Bogotá, 68 pp.
  67. Rivero, J.A. (1963) Five new species of Atelopus from Colombia, with notes on other forms from Colombia and Ecuador. Caribbean Journal of Science, 3 (2–3), 103–124.
  68. Romero-Garcia, J., Clavijo-Garzon, S. & Bernal, M.H. (2015) The advertisement call of three highland endemic species (Anura: Craugastoridae) from the Andean mountains of Colombia. Zootaxa, 4007 (2), 298–300. https://doi.org/10.11646/zootaxa.4007.2.14
  69. Saboyá-Acosta, L.P. & Urbina-Cardona, J.N. (2023) Current State of knowledge of Paramos amphibians in Colombia: Spatio temporal trends and information gaps to be strengthened for effective conservation. Tropical Conservation Science, 16, 1–23. https://doi.org/10.1177/19400829231169984
  70. Sánchez-Vialas, A., Miñarro, M., Padial, J.M. & De la Riva, I. (2023) Taxonomic reassessment and nomenclatural status of Niceforonia dolops and Hylodes philippi (Amphibia: Anura: Strabomantidae). Zootaxa, 5330 (1), 117–125. https://doi.org/10.11646/zootaxa.5330.1.6
  71. Sarmiento, C. & León, O. (2015) Transición bosque–páramo. Bases conceptuales y métodos para su identificación en los andes colombianos. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Bogotá, 156 pp.
  72. Sueur, J., Aubin, T. & Simonis, C. (2008) Seewave: A free modular tool for sound analysis and synthesis. Bioacoustics, 18, 213–226. https://doi.org/10.1080/09524622.2008.9753600
  73. Torres Constante, K.O. (2020) Systematics, phylogeny, and X-ray microtomography of a new amphibian species of the genus Pristimantis from Cordillera del Cóndor, Ecuador (Anura: Strabomantidae). Universidad de Investigación de Tecnología Experimental Yachay, Escuela de Ciencias Biológicas e Ingeniería, Urcuquí, 47 pp.
  74. Venegas, P.J., Barboza, A.C., de la Riva, I. & Padial, J.M. (2018) A new species of Phrynopus from the northeastern Andes of Peru, its phylogenetic position, and notes on the relationships of Holoadeninae (Anura: Craugastoridae). Zootaxa, 4446 (4), 501–524. https://doi.org/10.11646/zootaxa.4446.4.5
  75. Watters, J.L., Cummings, S.T., Flanagan, R.L. & Siler, C.D. (2016) Review of morphometric measurements used in anuran species descriptions and recommendations for a standardized approach. Zootaxa, 4072 (4), 477–495. https://doi.org/10.11646/zootaxa.4072.4.6
  76. Yánez-Muñoz, M.H., Altamirano-Benavides, M., Cisneros-Heredia, D.F. & Gluesenkamp, A.G. (2010) Nueva especie de sapo andino del género Osornophryne (Amphibia: Bufonidae) del norte de Ecuador, con notas sobre la diversidad del género en Colombia. ACI Avances en Ciencias e Ingenierías, 2 (3), B46–B53. https://doi.org/10.18272/aci.v2i3.44
  77. Zumel, D., Buckley, D. & Ron, S.R. (2022) The Pristimantis trachyblepharis species group, a clade of miniaturized frogs: description of four new species and insights into the evolution of body size in the genus. Zoological Journal of the Linnean Society, 195, 315–354. https://doi.org/10.1093/zoolinnean/zlab127

How to Cite

Pisso-Florez, G.A., Cardona-Giraldo, A., Ramírez-Chaves, H.E., Liévano-Bonilla, A.F., Mazepa, G.O. & Mulder, K.P. (2026) A new species of Niceforonia (Anura: Craugastoridae) from the Colombian Massif, southwestern Colombia. Zootaxa, 5881 (3), 301–326. https://doi.org/10.11646/zootaxa.5881.3.1