flUln
S.~f'!l!~ ~ UlNA from lri'lll..din. producin.~. eeu
1
ID~gl~1i\ CL'~ i~ Cna-'(},u 01' IfilN 11\.
r · · I ,'\.
EVE1:lKIl IlI1XavlKIl E ivrn TO ouvoxo TWV
TEXVlKWV rtou Xpr,OlilOrrOlEl 0 av8pwrroc; Yla
va ErrEIl~El OTO YEVETlKO UAlKO.
LTOXOl (2):
~L KOTavor,or, TWV uuornpltov tr,<; ~w~C; KaL Tr,<; E~EAl~r, e; rtcvco OIr] rr,
2, ~Ei\Tlwall Tile; uYEiac; Kal TOU TpOrrQU oLa~iwar]C; tWV av8pwrrwv usou uno tr,C; EcI>ap(.loyEc; tr,C;
ornv urrpucri, ern VEWPVia KQl OTI1V «tnvotpodilo
CI tcC ~-a~;8 b~ ,EcafN ~ ," . de nlJ-n1 ~i~ ti
iO
Clga.V~9 ~jto~ ~
CJa~V.fj~e .t:tyooR~ ~ 11d~ IlU~ E1800
•
•
". , . - lie. '. ." I .' ·11, ' .. ' toe ' .. '" .··t .. ' Ie : .' _
~.,". . ' .. ",,' . . ..
·aJto~oVWVOU~E OAlKO DNA cno Evav opvcviouo -to DNA K6~ETal ~E rtsptopurnxec EVOovouKAEaoEC;
eTa KO~~Eva t~~~aTa DNA evdivovrm ~E Evav ¢>opea KAwvorrOll1CJI1C; ·Aa~~avoU~E to cvcouvouccuevo DNA
• IlEtaOXflllatlOIlOe; tOU ~aKtflPlaKOU xurrcpou
• ErrlAEyoVtal ru «rrrcpu-Eevtorsc rrou EXOUV rrpOOAa~El to uvnouvdunouevo DNA ·Ka8E ~aKt~plo rrou EXEl rrpooAa~El DNA rroAAarrAaola~Etal «n Snuiouovel Ilia unoudn rrou cnorsxsl Evav ~aKtflPlaKo KAWVO
·IlE uurov tOV rpono EXOUV rrapax8Ei XlAlaoEe; KAWVOl, ola<l>opEtlKOi IlEta~U roue
Mia VOvlolwllaTlK~ ~l~Al08~Kr] ITEPlEXEl CAO TO
t5 I I I
YOVL .. uouo EVOC; opvovtouou
:.:~. Ta cncpclrnr« OTOlXEia Yla Til Squioupvic ~la<; VOVl{jlw~aTlK~<; ~l~Al08~KI1<; slvm (5):
~::. Ev~ulla: ITEPlOPlOTlKE<; Ev6ovOUKAEcioE<; «n DNA [jEo~aall
?, cj>OpE(C;: <PaYOl ~ ITA.ao~l6la ~aKTl1piwv (~E vovloto av8EKTlKoTIlTac; OE aVTl~loTlKa)
:J, paKt~pla: xwpic; rrAaa~iola
/:._ DNA rtou Sa J.lETa<pep8El
1;-"· :"1-..
... ): .'
. .," ~ ,
Xl1lllKEC; OUOlEC;: au,'-,avouv Til ularrEpaTOTIlTa TWV
I , r:t. '
KUTTaplKWv TOlXWJ.laTWv TOU pmctnpiou
~ I " 0
[] -TOUe;, aVWtEpOUC; EU KapUWTLKOUC; opvcviououc _ E
'''' 'A '6 'A
~ETaypa'"YOVTal o,a ro vovic La OE Q,QUe; roue
KUTTapLKOUe; TUITQUC;. Orov 8Ei\OU~E va KAWVOITOl~aOU~E vovlotu rn ortoio EKcIlpa~ovTal OE oUVKEKplllEva KUTTapa (oLaciloporroLIl ~Eva), rots Ka1"aOKEUa~oUIlE Tlt; eDNA PlP,Al09~KEC;.
o Mia eDNA ~l~A108~KI1 ITEPLEXEl uvtivpcdic TWV mRNA OAWV TWV vovrouov nou EKcIlpa~ovTal OTa
" I I
KUTTapa KCll aUYKEKplllEva TWV WPL~WV mRNA,
apa 1l0VO TWV E~wviwv.
H u~pl6orroir)al1 TWV VOUKAE"lKWV O~EWV XpF1(Jl~OrrolEtTal vux TI1V aviXVEUOIl KAWVWV Tile; YOvuSlwllaTlK~C; ~ eDNA ~l~Al08~KI1C;
TTG":'A' G' ··G·.··G·····G·:·· '.'
.' ._ .. -.". " . "..' '.' . " .' '.
. . .
. '." . ,'. " ..... -, '. .... . '." -.' ",
D H uppl6onol.,a., XPl1olll0nolElt"ul Vla (3):
1. va EVTOITlOTOUV OE ~la VOvlolw~aTlK~ ~l~Al08~KI1 rtotot KAWVOl ITEPlEXQUV OAoKAllpa YOVlOlU
2. va EVT011l0TOUV OE ~la VOVlOlw~aTlK~ ~l~Alo8~KI1 OUVKEKpl~Eva YOVLOla
3. va EVTOITlOTOUV ern eDNA ~l~Al08~ Kr) OUVKEKpl~Eva YOVLOlU
H aAUalOWT~ uvtlopcon rroi\u~Epaor]<; (peR) enrrpertst lOV EITlAEKTLKO rroAAarrAaaLaa~6 QAAIlAouXLwvDNA
HO' AA ' , ~ •
[~~J_ 11 ~LOU PY La no _ _WV QVt"LVP'Q'¥WV evoc
t"1l~llal"OC; DNA elvm crtcpnlrnrn npourtofleon VLa
~' , ~' ,
til ~EI\ETI1 EV0C; YOVLuLOU KaL YLa TI1V rrapaywYI1 tile;
npurrelvnc rrou KWOlKOT(OlEl.
[-j H Il,E906oC; aAual6wt"~C; avt"l6paal1C; noAuIlEpaal1C;, ~ac; EITlTPEn:El va aVTlypa4Jou~E EITlAEKTlKa, EKaTO~IlUpla ct>OPE<;, ElOlKE<; ai\i\I1AouXlEe; DNA crto Eva OUV8ETO ~Ely~a ~OPlWV DNA xWple; Til OlaOlKaOla Tile; Ki\WVOTtOlI1011C;.
......... -
IFor~iITI~llf8rl(cI
"
IPlFlllllllle~r Bac.~r'd
Extend
First cycle
l~~' e- _I C- '0-;- n-';- d
- 'ill l _,' _, ~_ ~_
~ - -
C~lc:llql
JI -,..." V
'Th'ilrcJI cylc III Ie!
· . :'"" ..
